USING THE RESAZURIN TEST TO ASSESS THE SURVIVAL OF MRC-5 CELLS EXPOSED TO COAL-ROCK DUST PARTICLES
Authors:
Type:
Сonference abstract
Published:
16.02.2023
Subject area:
UDK 66 Химическая технология. Химическая промышленность. Пищевая промышленность. Металлургия. Родственные отрасли
Language:
Russian
Keywords:
rezazurin, rezarufin, krasitel' alamarBlue, ugol'no-porodnye chasticy, RM10, MRC-5
Abstract (English):
Cel': ocenit' primenimost' metoda fluorescentnogo vyyavleniya produkta mitohondrial'nogo vosstanovleniya rezazurina – rezarufina dlya ocenki zhiznesposobnosti kul'tury fibroblastov legkih MRC-5 eksponirovannyh pylevymi chasticami ugol'noporodnogo proishozhdeniya ot 10 mkm do 100 nm. Nakoplenie produkta reakcii proishodit lineyno, chto otrazhaet biohimicheskoy aktivnost'yu kletok. Dlya kazhdoy proby byli polucheny uravneniya vida y=ax+b, gde koefficient «a» proporcionalen aktivnosti i chislu zhivyh kletok. V to zhe vremya ryad uglerodnyh produktov mozhet iskazhat' rezul'taty.
Cel': ocenit' primenimost' metoda fluorescentnogo vyyavleniya produkta mitohondrial'nogo vosstanovleniya rezazurina – rezarufina dlya ocenki zhiznesposobnosti kul'tury fibroblastov legkih MRC-5 eksponirovannyh pylevymi chasticami ugol'noporodnogo proishozhdeniya ot 10 mkm do 100 nm. Nakoplenie produkta reakcii proishodit lineyno, chto otrazhaet biohimicheskoy aktivnost'yu kletok. Dlya kazhdoy proby byli polucheny uravneniya vida y=ax+b, gde koefficient «a» proporcionalen aktivnosti i chislu zhivyh kletok. V to zhe vremya ryad uglerodnyh produktov mozhet iskazhat' rezul'taty.
Keywords:
rezazurin, rezarufin, krasitel' alamarBlue, ugol'no-porodnye chasticy, RM10, MRC-5
rezazurin, rezarufin, krasitel' alamarBlue, ugol'no-porodnye chasticy, RM10, MRC-5
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