GLYCOMACROPEPTIDES AS INDICATOR OF FAKE SKIM MILK POWDER
Abstract and keywords
Abstract (English):
Milk powder is a popular ingredient in various branches of the food industry. However, skim milk powder is often made of whey, which classifies as food fraud. Glycomacropeptide analysis can detect whey in milk powder. The biological and functional properties of glycomacropeptides depend on glycosylated heterogeneous carbohydrate chains. The colorimetric and fluorimetric method of detecting glycomacropeptide analysis relies on detecting sialic acid. The article describes the advantages and disadvantages of such methods of glycomacropeptide tests as polyacrylamide gel or capillary electrophoresis, as well as immunological, biosensor, and chromatographic methods. A universal glycomacropeptide test that could detect whey in milk powder remains an urgent task that requires further research and practical implementation.

Keywords:
skim milk powder, glycomacropeptides, adulteration, whey, research methods
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References

1. El'chaninov, V. V. Nomenklatura i svoystva belkov moloka korovy (Bos taurus): monografiya / V. V. El'chaninov. – Barnaul: Izd-vo Alt. gos. un-ta, 2022. – 300 s.

2. Sharma, N. Chemical and functional properties of glycomacropeptide (GMP) and its role in the detection of cheese whey adulteration in milk: a review / N. Sharma [et al.] // Dairy Science and Technology. 2013. № 93(1). R. 21–43. https://doi.org/10.1007%2Fs13594-012-0095-0

3. Eigel, W. Nomenclature of proteins of cow's milk: fifth revision / W. Eigel [et al.] // Journal of Dairy Science 1984. Vol. 67. Iss. 8. P. 1599–1631. https://doi.org/10.3168/jds.S0022-0302(84)81485-X

4. Koning, P. A method for the detection of small percentages of whey powder in milk powder / P. Koning, J. Elisses, H. De Vries // Netherlands milk and dairy journal. 1966. № 20(3). P. 203–212.

5. Neelima. Direct estimation of sialic acid in milk and milk products by fluorimetry and its application in detection of sweet whey adulteration in milk / Neelima [et al.] // Journal of Dairy Research. 2012. № 79(4). P. 495–501. https://doi.org/10.1017/s0022029912000441

6. Fukuda, S. Aplicação do método da ninidrina ácida como teste de “screening” de plataforma para a detecção da adição de soro ao leite / S. Fukuda, S. Roig, L. F. Prata // Ciênc Tecnol Aliment. 1996. №. 16. P. 52–56.

7. Nakano, T. Determination of sialic acid by the thiobarbituric acid reaction in sweet whey and its fractions / T. Nakano, L. Ozimek // Journal of Agricultural and Food Chemistry. 1999. №. 47. P. 2613–2616. https://doi.org/10.1021/jf981077y

8. Galindo-Amaya, L. L. Standardization of glycomacropeptide detection with SDS–PAGE as a milk adulteration index / L. L. Galindo-Amaya, E. Valbuena- Colmenares, E. Rojas-Villarroel // Rev Cient. (Maracaibo). 2006. Vol. 16, № 3. P. 308–314.

9. Recio, I. Detection of rennet whey solids in UHT milk by capillary electrophoresis / I. Recio [et al.] // International Dairy Journal. 2000. Vol. 10. Iss. 5–6 P. 333–338. https://doi.org/10.1016/S0958-6946(00)00076-5

10. Van Hooydonk, A. A rapid and sensitive high-performance liquid chromatography method of following the action of chymosin in milk / A. Van Hooydonk, C. Olieman // Netherlands milk and dairy journal. 1982. Vol. 36. P. 152–158.

11. Kawasaki, Y. pH-dependent molecular-weight changes of kappa-casein glycomacropeptide and its preparation by ultrafiltration / Y. Kawasaki [et al.] // Milchwissenschaft. 1993. №. 48. P. 191–196.

12. Chávez, N. A. Detection of bovine milk adulterated with cheese whey by western blot immunoassay / N. A. Chávez [et al.] // Food and Agricultural Immunology. 2008. №. 19. P. 265–272. https://doi.org/10.1080/09540100802381042

13. Martín-Hernández, C. Immunochromatographic lateral-flow test strip for the rapid detection of added bovine rennet whey in milk and milk powder / C. Martín-Hernández [et al.] // International Dairy Journal. 2009. Vol. 19. P. 205–208. https://doi.org/10.1016/j.idairyj.2008.10.016

14. Haasnoot, W. Spreeta-based biosensor immunoassays to detect fraudulent adulteration in milk and milk powder / W. Haasnoot, G. R. Marchesini, K. Koopal // Journal of AOAC International. 2006. №. 89. P. 849–855.

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